Redox Targets for Phosphine–Boranes

Understanding the complex mechanisms underlying redox-mediated biological processes is a fundamental pillar of cellular biology. We describe identification and quantification disulfide formation reduction in response to phosphine–borane complexes. illustrate specific cysteine effects novel bis(3-propionic acid methyl ester) phenylphosphine–borane (PB1) on cultured 661W cells. A total 1073 unique protein fragments from 628 proteins were identified quantified, which 13 found be statistically significant comparison control Among Notch1, HDAC1, UBA1, USP7, subunits L4 L7 60S ribosomal subunit, all are involved redox or cell death-associated pathways. Leveraging ability tandem mass tagging spectrometry provide quantitative data an exploratory manner provides insight into effect PB1 other compounds may have redoxome.